Figure 6.

Addition of IL-21 during expansion of TCR-transduced PBL results in T cells with high CD62L expression. CD62L expression after polyclonal feeder expansion of WT1 TCR-transduced PBL, gated on eGFP⁺ transduced cells for (a) and (b), or eGFP⁺ CD62L⁺ cells for (c). (a) Expression (left) and MFI (right) for CD62L for a representative PBL donor (of a total of 5 donors). Percentage of CD62L⁺ cells (b) and MFI for CD62L within these cells (c). Individual values and mean ± SD of 5 donors is shown. Results were compared using Kruskal–Wallis test followed by Dunn’s multiple comparisons test. p-value < 0.05 (*), p-value < 0.01 (**)