Abstract
The distribution of copper and zinc among soluble proteins in liver from normal slaughter cattle was examined after gel filtration of the proteins. Gopper- and zinc-binding proteins were mainly separated into three fractions. Varying amounts of zinc were eluted in a fourth fraction of molecular weight less than 2,000. A clear relationship was noted between the amount of copper bound to the low molecular weight fraction (m.w. ~ 10,000) and the total liver zinc concentration. The high molecular weight protein fraction (m.w. > 65,000) dominated in liver with zinc concentrations below 40 µg/g wet weight and total copper concentrations from 16 to 240 µg/g, while in liver with zinc concentrations above 40 µg/g and copper concentrations ranging from 20 to 107 µg/g, the low molecular weight metallothionein-like fraction dominated.
Keywords: mastitis, Staphylococcus aureus, nuclease, antinucleases
Sammendrag
Løslige kobber- og sinkholdige leverproteiner fra storfe ble fraksjonert ved hjelp av gelfiltrering. Prøvene kom fra nörmale slaktedyr og ble plukket ut for å dekke et størst mulig konsentrasjonsområde av de to metallene. De løslige kobber- og sinkholdige proteinene ble separert i 3 fraksjoner med følgende omtrentlige molekylvekter: fraksjon I <65.000, fraksjon II 35.000 og fraksjon III 10.000. Varierende mengder sink ble eluert i en fjerde fraksjon med molekylvekt >2.000. Det var en tydelig relasjon mellom innholdet av kobber i fraksjon III og konsentrasjonen av sink i leveren. Prøvene ble derfor delt inn i 2 grupper, med sinkkonsentrasjoner i leveren over og under 40 µg/g våtvekt. I prøver med sinknivåer under 40 µg/g og kobbernivåer fra 16 til 240 µg/g dominerte fraksjon I. I disse prøvene ble ikke sink påvist i fraksjon III. I prøver med sinknivåer over 40 µg/g og kobbernivåer fra 20 til 107 µg/g ble det funnet signifikant høyere innhold av kobber og sink bundet til de metallothionin-lignende proteinene i fraksjon III.
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