Abstract
Experimental staphyloenterotoxicosis was produced in minks by oral administration of mink feed containing 5 or 200 µg of purified enterotoxin A per test animal. The animals became very exhausted after the ingestion of toxin. Vomiting was observed in two of seven minks of the lower toxin group with a latent period of 2.5 to 4.0 h. The higher toxin concentration caused vomiting in four of seven test animals with a latent period of 2.0 to 2.5 h. Vomitus was accompanied by strong salivation. Poor appetite was observed in four of seven minks having ingested 5 µg of SEA, and 200 µg caused total loss of appetite in all the test animals. After a test period of 22 h all the animals but one had normal appetite. Diarrhoea was prominent in three of seven minks with the low toxin concentration and in all with the high toxin concentration. Statistically significant haemato-logical changes compared to the control group were an increase in neutrophil count and a decrease in lymphocyte count in the high toxin group. Significant changes in the blood chemical data were an increase in blood urea nitrogen with 200 µg of SEA and a decline in the cholesterol level in both toxin groups.
Keywords: enterotoxicosis, minks, experimental staphylococcal intoxication, mink feed, bacterial toxins
Sammanfattning
Experimentell stafyloenterotoxikos framkallades på mink genom utfodring med ett minkfoder tillsatt med 5 resp. 200 µg stafylokockenterotoxin A (SEA) per djur. Minkarna visade sig mycket slöa efter toxinupptagandet; efter 2,5–4,0 timmar iakttogs kräkningar hos två av de sju minkar, som fått den lägre toxindosen. Den högre toxindosen gav efter 2,0–2,5 timmar upphov till kräkningar hos fyra av sju försöksdjur i den andra gruppen. Kräkningarna åtföljdes av en stark salivation. Hos fyra av de sju minkar, som fick 5 µg SEA var aptiten nedsatt; 200 µg SEA orsakade fullständig inappetens hos samtliga sju djur i den andra gruppen. Efter 22 timmar hade samtliga djur, sä när som på ett, normal aptit. En kraftig diarré förekom hos tre av sju minkar på låg och hos alla på hög toxinkoncentration. En statistisk signifikant ökning av neutrofiler och minskning av lymfocyter förekom i blodet hos gruppen med hög toxindosering. I samma grupp förelåg en ökning av blodets ureakväve medan bägge toxingrupperna visade en sänkning av blodets kolesterolvärden.
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