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. 1980 Sep 1;21(3):318–323. doi: 10.1186/BF03546862

A Comparative Investigation of Antibody to Swine Vesicular Disease Virus Using Counter Immunoelectrophoresis, Serum Neutralization and Double Immunodiffusion Tests

En sammenlignende undersøgelse for antistof mod swine vesicular disease virus ved hjælp af counter immunoelektroforese, serum neutralisations- og dobbelt immunodiffusionstest

K J Sørensen 1,✉
PMCID: PMC8317763  PMID: 6255777

Abstract

A counter immunoelectrophoresis test (GIET) for assay of antibody to swine vesicular disease virus (SVDV) is described and compared with the serum neutralization test (SNT) and the double immunodiffusion test (DIDT). Using a preparation of complete or empty virus particles as antigen the GIET was found to be more sensitive than the DIDT. The GIET is rapid and economic, and although less sensitive than the SNT it was suggested as an initial large scale screening test for SVD antibody.

Keywords: SVD antibody, pig, counter immunoelectrophoresis, serum neutralization, double immunodiffusion

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References

  1. Brooksby J B. Swine vesicular disease: History of the disease. Vet. Rec. 1975;95:108. [Google Scholar]
  2. Croxton, F. E.: Elementary Statistics with Applications in Medicine and the Biological Sciences. Dover Publications, Inc., New York 1959, 209–244.
  3. Daugherty, R. M.: Animal titration techniques. In R. J. G. Harris (ed.): Techniques in Experimental Virolotgy. Acad. Press Inc., New York 1964, 169–220.
  4. Golding S M, Hedger R S, Talbot P, Watson J. Radial immunodiffusion and serum-neutralization techniques for the assay of antibodies to swine vesicular disease. Res. vet. Sci. 1976;20:142–147. doi: 10.1016/S0034-5288(18)33445-3. [DOI] [PubMed] [Google Scholar]
  5. Graves, J. H.: Serological relationship of swine vesicular disease and Coxsackie B5 viruses. Nature (Lond.) 1973, 245, 314–315. [DOI] [PubMed]
  6. Hendrie E W, Watson J, Hedger R S, Rowe L W, Garland Y J. Swine vesicular disease: Continuing serological surveys of pigs presented for slaughter in the United Kingdom. Vet. Rec. 1977;100:363–365. doi: 10.1136/vr.100.17.363. [DOI] [PubMed] [Google Scholar]
  7. Hendrie E W, Baker K, Hedger R, Davies G, Richards M S. Swine vesicular disease: Serum surveys 5 and 6. Vet. Rec, 1978;102:126–127. doi: 10.1136/vr.102.6.126. [DOI] [PubMed] [Google Scholar]
  8. Jansen, P. & G. Maass: Nachweiss präzipitierende Antikörper gegen Coxsackie virus B5 mit Hilfe der Acetatfolienelektrophorese. (The demonstration of precipitating antibodies against Coxsackie virus type B5, using acetate foil electrophoresis). ZW. Bakt., I. Abt. Orig. A 1973, 224, 309–315. [PubMed]
  9. Pereira H G, Rowe L, Baber D. Use of double immunodiffusion (Ouchterlony) test for the diagnosis of swine vesicular disease. Res. vet. Sci. 1976;20:139–141. doi: 10.1016/S0034-5288(18)33444-1. [DOI] [PubMed] [Google Scholar]
  10. Schmidt N J, Dennis J, Ftommhagen L H, Lenette E H. Serological reactivity of certain antigen obtained by fractionation of Coxsackie viruses in cesium cloride density gradients. I. Immunology. 1963;90:654–662. [PubMed] [Google Scholar]
  11. Sullivan E J, Rosenbaum M J. Methods for preparing tissue culture in disposable microplates and their use in virology. Amer. J. Epidem. 1967;85:424–437. doi: 10.1093/oxfordjournals.aje.a120704. [DOI] [PubMed] [Google Scholar]
  12. Sørensen K J. A comparative electrophoretic examination of swine vesicular disease virus and Coxsackie B5 virus. Arch. Virol. 1977;53:235–241. doi: 10.1007/BF01314667. [DOI] [PubMed] [Google Scholar]
  13. Watson J, Hedger R S. Swine vesicular disease: A serological survey of pigs presented for slaughter. Vet. Rec. 1974;95:535. doi: 10.1136/vr.95.23.535. [DOI] [PubMed] [Google Scholar]

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