Skip to main content
NCBI home page
Acta Veterinaria Scandinavica logoLink to Acta Veterinaria Scandinavica
. 1980 Mar 1;21(1):124–133. doi: 10.1186/BF03546907

Experimental Maedi Infection in Sheep

2. Antibody Response

Experimenten maedi infektion hos får. 2. Bildningen av antikroppar. Alla fyra får som inokulerats via luftvägarna med 7×106 TCID50 av maedi M88 stammen påvisade komplement bindande (CF) antikroppar inom tre månader efter inokulationer, och en gradvis ökning av CF-titern upptäcktes under det första året. Under det påföljande året har CF antikropptitern trots en ringa fluktuation bibehållits och varierat mellan 64 og 256. Virusneutraliserande aktivitet mot maedi M88 stammen upptäcktes i sera av alia intrapulmonalt inokulerade får inom åtta månader efter inokulationen. Titrarna har bibehållits eller ökat lindrigt. Nivån av titrarna, som släckte sig från 8 till 256, har klart varierat mellan individerna.

Nabila Gazia 1,✉, Inger Wegger 1
PMCID: PMC8317773  PMID: 7386321

Abstract

All 4 sheep inoculated via the respiratory tract with 7×106 TCID50 af maedi M88 strain developed complement fixing (CF) antibodies within 3 months after inoculation, and a gradual rise in CF titers was found during the first year. The antibody titers have been maintained, though with some fluctation, through the following year, and the titers vary from 64 to 256. Virus neutralizing activity against maedi M88 strain was detected in the sera of all intrapulmonarily inoculated sheep within 8 months after inoculation. Titers have been maintained or have slightly increased. The level of titers, ranging from 8 to 256, was clearly different between individual sheep.

One of the 4 sheep inoculated intracerebrally with 5×105 TCID50 of maedi M88 strain developed CF antibodies 1 month after inoculation, but no neutralizing antibodies until death 11 months after inoculation. The rest of the intracerebrally inoculated sheep displayed no evidence of CF or neutralizing antibodies within 18 months after inoculation in spite of numerous virus isolations from peripheral blood leukocytes. The absence of antibodies might perhaps be attributed to phenomena such as differences in tropism, provirus state, immunological tolerance and size of inoculum.

One sheep hyperimmunized with repeated s.c. and i.v. injections of maedi M88 strain developed high CF antibody titers but lower neutralizing antibody titers.

The 2 uninoculated control sheep developed no CF or neutralizing antibodies within 18 months after inoculation.

Keywords: acetoacetate, β-hydroxybutyrate, blood glucose, clinical trial, dairy cows, Finland

Full Text

The Full Text of this article is available as a PDF (942.0 KB).

Footnotes

This work was supported by the Finnish Academy.

References

  1. De Boer G. F. Antibody formation in zwoegerziekte, a slow infection in sheep. J. Immunol. 1970;104:414–422. [PubMed] [Google Scholar]
  2. Gudnadottir, M.: Host-virus interaction in maedi infected sheep. In Lung Tumours in Animals. Proc. 3rd Int. Gonf. Cancer, Perugia 1966, 381–391.
  3. Gudnadottir M. Visna-maedi in sheep. Progr. med. Virol. 1974;18:336–349. [PubMed] [Google Scholar]
  4. Gudnadottir M., Kristinsdottir K. Complement-fixing antibodies in sera of sheep affected with visna and maedi. J. Immunol. 1967;98:663–667. [PubMed] [Google Scholar]
  5. Gutlip, R. C., T. A. Jackson & G. A. Laird: Immunodiffusion test for ovine progressive pneumonia. Amer. J. vet. Res. 1977, 38, 1081–1084. [PubMed]
  6. Haase A. T., Stowring L., Narayan O., Griffin D., Price D. D. Slow persistent infection caused by visna virus: Role of host restriction. Science. 1977;195:175–177. doi: 10.1126/science.188133. [DOI] [PubMed] [Google Scholar]
  7. Narayan O., Griffin D. E., Silverstein A. M. Slow virus infection: Replication and mechanisms of persistence of visna virus in sheep. J. infect. Dis. 1977;135:800–806. doi: 10.1093/infdis/135.5.800. [DOI] [PubMed] [Google Scholar]
  8. Pálsson, P. A.: Maedi and visna in sheep. In Kimberlin, R. H. (ed.): Slow Virus Diseases of Animals and Man, Frontiers in Biology. Vol. 44. North Holland Publishing Co., Amsterdam and Oxford 1976, 17–43.
  9. Petursson, G.: Comment/Chapter 3. In ter Meulen, V. & Katz, M. (ed.): Slow Virus Infections of the Central Nervous System. Springer-Verlag, New York, Heidelberg, Berlin 1977, 71–72.
  10. Petursson G., Nathansson N., Georgsson G., Panitch H., Palsson P. A. Pathogenesis of visna 1. Sequential virologic, serologic and pathologic studies. Lab. Invest. 1976;35:402–412. [PubMed] [Google Scholar]
  11. Sihvonen L., Estola T., Tuomi J. Experimental maedi infection in sheep. 1. Detection of virus, cliniical course, histopathology. Acta vet. scand. 1980;21:113–123. doi: 10.1186/BF03546906. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Terpstra C., De Boer C. F. Precipitation antibodies against maedivisna virus in experimentally infected sheep. Arch. ges. Virus-forsch. 1973;43:52–62. doi: 10.1007/BF01249348. [DOI] [PubMed] [Google Scholar]

Articles from Acta Veterinaria Scandinavica are provided here courtesy of BMC

RESOURCES