Abstract
Serological typing of Group-B streptococci isolated on different occasions during the course of natural intramammary infections revealed no shifts between polysaccharide types, but showed that polysaccharide antigens and the protein Ibc antigen may be lost and regained. Pure X-strains may arise from strains previously typed as IIIX or III, or from non-typeable strains, and possibly from Ibc, Ia and Ib strains as well. Such strains may later revert to their original types.
Keywords: Group-B streptococci, serological types, variations of type antigens
Sammendrag
Typebestemmelsesresultater fra 16 af “X-besaetningerne” analyseredes nøjere med henblik på påvisning af art ag hyppighed af antigenvariation.
I de 16 besætninger, der kunne opdeles i 4 kategorier (Tabel 1), fandtes 588 B-str. inficerede kirtler.
Retypisering (= gentagen typebestemmelse, når en kirtel viste sig fortsat inficeret ved en senere besætningsundersøgelse) blev foretaget for isolater fra 209 kirtler; 123 af disse udskilte fortsat B-str. af samme type, medens 86 kirtler viste typeskift ved retypisering. For de 86 varierende kirtler forelå ialt 149 typebestemmelser. To på hinanden følgende undersøgelser viste samme type i 46 tilfælde, medens 103 viste variation. Tretten kirtler reverterede efter variation til den oprindelige type. Fire kirtler udskilte B-str. af 3 typer.
Variationernes art, antal og udbredelse fremgår af Tabel 2. Variationen III⇆IIIX var hyppigst forekommende (51/103) og mest udbredt (9/16).
Tab af polysakkaridantigen III sås i 5 besætninger i ialt 17 tilfaelde, og omvendt kunne isolater fra X eller NT kirtler (4) ved senere undersøgelse vise sig at have III antigenet. Variationen X⇆NT var almindelig (19/103). Andre antigenvariationer forekom sjaeldent (se Tabel 2) i dette materiale,
Undersøgelsen viste, at der ikke sker skift mellem polysakkaridtyper, men at såvel polysakkaridantigener som Ibc proteinantigenet er variabelt. X-stammer kan hidrøre fra stammer tidligere typebestemt til IIIX, III eller NT og antagelig også fra Ibc, Ia og Ib stammer.
Det konkluderes, at påvisning af B-str. af fiere typer i en besætning ikke umiddelbart viser, at en sådan besætning er blevet inficeret fra forskellige kilder.
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