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. 1979 Sep 1;20(3):313–320. doi: 10.1186/BF03546593

Rapid Method for Screening of Immunoglobulins in Porcine Fetuses, Using Rocket Immunoelectrophoresis

Application of an Interspecies Reaction Between Human and Porcine μ-Chain

Raket immunelektroforese som en hurtig metode til “screening” af immunglobuliner i grisefostre. Anvendelse af en interspecies reaktion mellem human og porcin μ-kæde

K Dalsgaard 1,2,3,✉, E Overby 1,2,3, J J Metzger 1,2,3, A Basse 1,2,3
PMCID: PMC8322912  PMID: 506870

Abstract

A method for screening of immunoglobulin levels in porcine fetuses is presented. The method is based on rocket electrophoresis. This method is sensitive, but when used for assaying immunoglobulins it is only semiquantitative. It was shown that sera from 11 hysterectomy-derived colostrum-deprived neonatal pigs from a normal gilt contained no IgM or IgA, but small amounts of IgG. Sera from 9 piglets isolated in the same way from a gilt infected with porcine parvovirus contained all 3 antibody classes. IgM was detected by an interspecies reaction using anti human μ-chain immunoglobulin.

Keywords: pig fetus, parvovirus, immunoglobulins, rocket electrophoresis

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Footnotes

This work was partly supported by the Commission of the European Communities (Swine fever programme).

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