Skip to main content
Acta Veterinaria Scandinavica logoLink to Acta Veterinaria Scandinavica
. 1979 Mar 1;20(1):10–15. doi: 10.1186/BF03546624

Blood-Volume Determination with Evans Blue Dye in Foals

Bloduolymsbestämning med Evans blue på föl

S G B Persson 1,, L-E Ullberg 1
PMCID: PMC8322919  PMID: 443134

Abstract

The rate of disappearance of Evans blue dye from the blood after an intravenous injection was studied in young foals, between 4 and 105 days of age. This was found to be age dependant, especially during the first month, the initial dye disappearance being much faster than in the adult horse. This would mean an overestimation of plasma volume, using the single sampling technique, of about 5% during the first day of life, i.e. negligible from a practical point of view considering the standard error of estimation.

The effect of exercise on the venous haematocrit was studied, too, in foals less than 3 months of age. There was a significant difference between mean values before and after exercise, and this difference seemed to increase with age, indicating an increasing erythrocytestoring capacity of the spleen. This means that even in young foals, the splenic function should be considered when determining the total blood volume from the plasma volume and the venous haematocrit.

Keywords: foal, plasma volume, dye-disappearance rate, total blood volume, splenic function

Full Text

The Full Text of this article is available as a PDF (600.1 KB).

References

  1. Ekman, L., S. G. B. Persson & L.-E. Ullberg: The levels of some blood constituents in standardbred horses during their first year of life. Proc. 1st int. Symp. Eq. Hematol. 1975, 289–296.
  2. Gregersen M I, Rawson R A. The disappearance of T-1824 and structurally related days from the blood stream. Amer. J. Physiol. 1942;138:698–707. doi: 10.1152/ajplegacy.1943.138.5.698. [DOI] [Google Scholar]
  3. Hald, A.: Statistical Theory with Engineering Applications. Wiley & Sons Inc., New York 1952.
  4. Persson, S. G. B.: On blood volume and working capacity in horses. Acta vet. scand. 1967, Suppl. 19, 1–189. [PubMed]
  5. Persson S G B. Value of haemoglobin determination in the horse. Nord. Vet.-Med. 1969;21:513–523. [Google Scholar]
  6. Persson, S. G. B., L. Ekman, G. Lydin & G. Tufvesson: Circulatory effects of splenectomy in the horse. I. Effect on red cell distribution and variability of haematocrit in the peripheral blood. Zbl. Vet.-Med. A, 1973 a, 20, 441–455. [PubMed]
  7. Persson, S. G. B., L. Ekman, G. Lydin & G. Tufvesson: Circulatory effects of splenectomy in the horse. II. Effect on plasma volume and total and circulating red-cell volume. Zbl. Vet.-Med. A, 1973 b, 20, 456–468. [PubMed]

Articles from Acta Veterinaria Scandinavica are provided here courtesy of BMC

RESOURCES