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. 2021 Jul 16;24(8):102870. doi: 10.1016/j.isci.2021.102870

Figure 2.

Figure 2

Effect of Dex on sperm cells at different time points post Dex administration

(A–C) (A) Experimental design showing the location of sperm at the time of injection and timing of sperm harvest. MA (log-intensity ratios [M-values] versus log-intensity averages [A-values]) plots depicting (B) effect of Dex (log2 fold changes control versus dexamethasone), (C) time after injection (log2 fold changes 7 days versus 3 hr) (7 days Dex n = 4 and controls n = 4, 3 hr Dex n = 3 and controls n = 4). TsRNAs are indicated by sequence identity for display only; each dot represents one small RNA. MA plot depicts log2 fold changes on the y axis and the expression level on the x axis (the higher the expression the further to the right). Statistically significantly changed small RNAs are highlighted in red q < 0.05.

(D) Relative expression of ArgCCT-2 as obtained by q-RT-PCR (cauda: Dex n = 4, controls n = 4, caput: Dex n = 4, controls n = 5; interaction F(1,13) = 6.34, p = 0.0257, treatment F(1,13) = 5.97, p = 0.0040, site of collection (F(1,13) = 12.15, p = 0.0296; cauda control versus cauda Dex t(13) = 3.42, p = 0.0274, cauda Dex verus caput Dex t(13) = 4.137, p = 0.007). Whiskers display minimum and maximum. ∗p < 0.05, mixed-effect model group effect of location (REML), ∗∗p < 0.01 multiple comparisons SIDAK corrected.