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. 2021 Jul 31;26(15):4658. doi: 10.3390/molecules26154658

Figure 3.

Figure 3

(A) Investigation of Raji cell apoptosis induced by DFMT with or without pretreatment with 0.02 wt% β-CD or 1 mM EGTA. (B) Flow cytometry time lapse fluorescence of calcium chelator Fluo-3AM after addition of nanoconjugates in DARA-based DFMT (DARA-MORF1 + HSA-(MORF2)10 (left panel) and Fab’DARA-based DFMT (Fab’DARA-MORF1 + HSA-(MORF2)10) (right panel). Red arrows indicate time of nanoconjugate addition to cell sample. (C) Confocal microscopy images of β-CD or EGTA treated Daudi cells compared to normal cells undergoing Fab’DARA-based DFMT. *** p < 0.001, ** p < 0.01, n.s. nonsignificant, by One-Way ANOVA and Tukey test.