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. 2021 Aug 3;26(15):4688. doi: 10.3390/molecules26154688

Table 1.

In this table, the main techniques employed for peptide purification and their interaction mechanisms are summarized.

Purification or Mechanism
Identification Method
Ultrafiltration Target peptide is separated from other species depending on their size
IsoElectroFocusing (IEF) Peptides are separated on the basis of their isoelectric point through an electric field and a pH gradient
Single-column chromatography Different modes of chromatography have been developed, depending on the chemical features of the analytes:

  • RP-LC: hydrophobic character

  • Ion-Exchange: charge

  • HILIC: hydrophilic character

  • Mixed-Mode: combination of two ligands on the same stationary phase with orthogonal interection mechanisms
Multicolumn chromatography Combination of two or more orthogonal chromatographic modes applied consecutively
MCSGP Same separation principles as single-column chromatography but with the use of two or more identical columns. The performance parameters increase due to internal recycling of impure fractions into the system.