Glycan-seq analysis of hiPSC and NPCs
(A) Binding of PE-labeled rBC2LCN to hiPSCs after differentiation to NPCs for 0 (red line), 4 (green line), and 11 days (blue line) was analyzed by flow cytometry.
(B) Binding of DNA-barcoded rBC2LCN to hiPSCs after differentiation to NPCs for 0 (red bar), 4 (green bar), and 11 days (blue bar) was analyzed by Glycan-seq. Data are shown as average ± SD of triplicates of the same sample. See also Figures S4–S6.