Fig. 4.

Identification of survival-related molecular subtypes in ESCC.

A. Screening of survival-related genes in GSE53625 (upper) and TCGA ESCC (lower) cohorts using Cox regression analysis. Risky genes were defined as genes with a hazard ratio (HR) > 1 whereas genes with HR < 1 were considered as protective genes (p < 0.05).

B. Overlapping of risky (upper) and protective (lower) genes identified in GSE53625 and TCGA ESCC cohorts.

C. Identification of representative marker genes using LASSO Cox regression analysis in GSE53625 cohort. Risky genes were colored in orange and protective genes were green.

D. S1/S2 subtype patients showed distinct OS differences in both GSE53625 (left) and TCGA ESCC (right) cohorts (Log-rank test, p < 0.05).

E. Submap analysis showing a significant correlation of classification among three ESCC cohorts. A p-value < 0.05 indicating a significant similarity.

F. The heatmap showing the biological pathway differences between S1 and S2 subtypes (Wilcoxon rank sum test, p <0.05).