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. Author manuscript; available in PMC: 2021 Oct 30.
Published in final edited form as: Science. 2021 Mar 25;372(6541):eabe9233. doi: 10.1126/science.abe9233

Figure 2. Intracellular localization of MHC-E in the presence and absence of Rh67 and UL40.

Figure 2.

(A) Intracellular localization of V5-taggegd Rh67 and UL40, Calnexin and MHC-E was determined in UL49.5-expressing, Mamu-E*01:03-transfected TRF by immunofluorescence assay using indicated antibodies. Nuclei were stained with DAPI. Cells shown below are highlighted. (B) Internalization of MHC-E was monitored by adding anti-MHC-E antibody for 1 hour at 37°C prior to fixation, permeabilization and co-staining for early endosomal antigen 1 (EEA1) or lysosome-associated membrane glycoprotein 1 (LAMP-1). Representative results are shown. (C) Boxplots show Manders’ Co-localization Coefficient (M2) calculated for co-localization of EEA1 (n=5 images) or LAMP1 (n=6 images) with MHC-E in Rh67 (left panel) and UL40 (right panel) expressing cells (Extended Date File 1). P-values are shown for two-sample Wilcoxon rank-sum tests comparing the degree of co-localization of EEA1 vs. LAMP1.