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. Author manuscript; available in PMC: 2021 Oct 30.
Published in final edited form as: Science. 2021 Mar 25;372(6541):eabe9233. doi: 10.1126/science.abe9233

Figure 4. Effect of Rh67 deletion on RhCMV/SIV vector CD8+ T cell response programming.

Figure 4.

Comparison of SIVgag-specific CD8+ T cell responses elicited by 68–1 RhCMV/SIVgag (RM 1.04, 1.05, and 1.06) versus ΔRh67 68–1 RhCMV/SIVgag (RM 44, 45, and 46) vectors. Peripheral blood CD8+ T cells from these RM inoculated with each of these vectors were assessed by flow cytometric ICS assay for responses to a mixture of 125 consecutive 15mer peptides comprising the SIVgag protein sequence (red; top panel), to individual MHC-E and MHC-II-restricted SIVgag supertopes (green and blue, respectively; top panel; Gag69 = Gag276–284, Gag120 = Gag482–490; Gag53 = Gag211–222, Gag73 = Gag290–301), and to each of 125 consecutive 15mer SIVgag peptides with any above threshold (≥ 0.05% after background subtraction) responses indicated by a box. Boxes are colored to reflect MHC restriction based on the ability to inhibit the response with the MHC-E blocking peptide VL9, the MHC-II blocking mAb G46–6, or the pan-MHC-I blocking mAb W6/32 (14). In the bottom panels, arrowheads indicate the 15mer peptides including the previously described MHC-E- and MHC-II-restricted supertopes (12).