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. 2021 Aug 4;8(4):ENEURO.0566-20.2021. doi: 10.1523/ENEURO.0566-20.2021

Figure 3.

Figure 3.

Cell proliferation and the survival of new neurons were reduced in the dentate gyrus of adult DG-NR1KO mice. A, Representative images visualizing Ki67+ (green) and DAPI-labeled (red) cells in the dentate gyrus of adult control and DG-NR1KO mice. Scale bar: 75 μm. B, Density of Ki67+ cells in the subgranular zone. C, Representative images showing DCX+ (green) and DAPI-labeled (red) cells in the dentate gyrus of adult control and DG-NR1KO mice. The images were maximum intensity projections of confocal Z stacks and formed by joining two overlapping images containing adjacent areas. Scale bar: 25 μm. D, Density of DCX+ cells. E, Representative images showing BrdU+ (green) and Prox1+ (red) cells in adult control and DG-NR1KO mice on 7 and 28 d after BrdU injections. Scale bar: 75 μm. F, Density of BrdU+ cells in the granule cell layer and subgranular zone. G, Proportion of BrdU+ cells expressing Prox1. H, Density of BrdU+/Prox1+ cells in the granule cell layer. I, Density of BrdU+/Prox1– cells in the granule cell layer. J, Survival rate of BrdU+/Prox1+ cells from 7 to 28 dpi. Density at 28 d after BrdU injection was divided by mean values of density at 7 d; *p < 0.05, **p < 0.01, ***p < 0.005, independent-sample t test, two tailed.