Fig. 2. Remifentanil effects on PrLC L5/6 pyramidal neuron GABAB-GIRK signaling.
a Self-administration and abstinence timepoints. b Baclofen-evoked currents (IBaclofen) from male (unfilled) and female (filled) PrLC L5/6 pyramidal neurons 14–21 days after saline (gray), 10–16-day remifentanil self-administration (blue), or 25–30-day remifentanil self-administration (orange). IBaclofen was no different in 10–16-day vs. 25–30-day saline males or females and thus were combined. IBaclofen was reduced in 10–16-day remifentanil males vs. saline males and 25–30-day remifentanil males, whereas 25–30-day remifentanil males showed an increase in IBaclofen vs. saline males. IBaclofen did not differ when comparing saline females with 10–16- or 25–30-day remifentanil females. A difference was observed between 10- and 16-day remifentanil males and females. c Representative IBaclofen traces in males (top) and females (bottom) across treatments. Representative scale bars: 50 pA/200 s. *p < 0.05, ***p < 0.001.