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. 2021 Jul 28;24(8):102910. doi: 10.1016/j.isci.2021.102910

Figure 1.

Figure 1

S. flexneri activates the gasdermin D response through caspase-1 but evades the caspase-11 response

(A–F) Indicated BMMs were primed with LPS (50 ng/mL) overnight and subsequently infected with S. flexneri (MOI 50) for 4 hr. Extracellular bacteria were eliminated by adding gentamicin (100 μg/mL) 1 hr after infection. Cytotoxicity was determined by LDH release assay (A and D), and IL-1β release was determined by ELISA (B and E). Western blots were probed with the indicated antibodies (C and F). GAPDH was used as a loading control.

Bar represents mean ± SEM. Data are representative of three independent experiments. Cytotoxicity and IL-1β levels were analyzed by one-way analysis of variance (ANOVA) followed by Tukey's multiple comparison test with a statistical threshold of p ≤ 0.05. Groups that do not share a letter are significantly different from each other, p ≤ 0.05.