Table 5.
Statistical analysis of the behavioral experiments in P21 and adult males
| Behavioral test | Sex | Age | Normal data? | Equal variance? | Test | Results |
|---|---|---|---|---|---|---|
| Open field test: total distance day 2 | M | P21 | Yes | Yes | One-way ANOVA |
F(2,72) = 5.017; p = 0.0091 Post hoc Tukey’s test: WTSGH vs WTMGH; p = 0.0063 WTSGH vs KO; p = 0.2796 WTMGH vs KO; p = 0.1468 |
| Open field test: total distance day 2 | M | P60 | Yes | Yes | One-way ANOVA | F(2,68) = 1.13; p = 0.329 |
| Open field test: intervals day 2: 0–5 min | M | P21 | No | Yes | Kruskal–Wallis |
H(2) = 9.354; p = 0.0093 Post hoc Dunn’s test: WTSGH vs WTMGH; p = 0.0079 WTSGH vs KO; p = 0.1711 WTMGH vs KO; p = 0.4797 |
| Open field test: intervals day 2: 5–10 min | M | P21 | Yes | Yes | One-way ANOVA | F(2,72) = 0.719; p = 0.491 |
| Open field test: intervals day 2: 10–15 min | M | P21 | Yes | Yes | One-way ANOVA | F(2,72) = 0.976; p = 0.382 |
| Open field test: intervals day 2: 15–20 min | M | P21 | Yes | Yes | One-way ANOVA | F(2,72) = 2.184; p = 0.12 |
| Open field test: intervals day 2: 0–5 min | M | P60 | Yes | Yes | One-way ANOVA | F(2,68) = 1.312; p = 0.276 |
| Open field test: intervals day 2: 5–10 min | M | P60 | Yes | Yes | One-way ANOVA | F(2,68) = 1.292; p = 0.2813 |
| Open field test: intervals day 2: 10–15 min | M | P60 | Yes | Yes | One-way ANOVA | F(2,68) = 0.13; p = 0.879 |
| Open field test: intervals day 2: 15–20 min | M | P60 | No | Yes | Kruskal Wallis | H(2) = 1.56; p = 0.4584 |
| Open field test: time in left day 2 | M | P21 | No | Yes | Kruskal Wallis | H(2) = 2.7579; p = 0.2518 |
| Open field test: time in left day 2 | M | P60 | No | Yes | Kruskal Wallis | H(2) = 3.2761; p = 0.1671 |
| 24 h activity: total | M | >P60 | Yes | Yes | One-way ANOVA | F(2,34) = 0.4831; p = 0.6210 |
| 24 h activity: light period | M | >P60 | Yes | Yes | One-way ANOVA | F(2,34) = 3.031; p = 0.0615 |
| 24 h activity: dark period | M | >P60 | Yes | Yes | One-way ANOVA | F(2,34) = 0.3135; p = 0.7330 |
| Elevated plus maze | M | P21 | Yes | Yes | One-way ANOVA | F(2,72) = 1.994; p = 0.144 |
| Elevated plus maze | M | P60 | Yes | Yes | One-way ANOVA |
F(2,68) = 6.879; p = 0.0019 Post hoc Tukey’s test: WTSGH vs WTMGH; p = 0.9893 WTSGH vs KO; p = 0.0042 WTMGH vs KO; p = 0.0138 |
| Social interaction | M | P21 | Yes | Yes | One-way ANOVA |
F(2,72) = 2.911; p = 0.039 Post hoc Dunnett’s test (all vs WT SGH): WTSGH vs WTMGH; p = 0.0382 WTSGH vs KO; p = 0.0771 |
The table includes the behavioral test analyzed, sex and age of the animals, data and variance distribution, statistical test used, and results obtained. M, Male.