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. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: Hepatology. 2020 Nov 3;73(3):1176–1193. doi: 10.1002/hep.31328

FIG. 6.

FIG. 6.

Vit E supplementation partially prevented steatosis in PCBP1Δhep mice. Mice were fed a 50-ppm iron diet or the same diet with 500 U of vit E for 16–18 days. (A) Decreased steatosis in PCBP1Δhep mice fed a vit E diet by H&E staining of liver sections. (B) Decreased plasma ALT in PCBP1Δhep mice fed a vit E diet. (C) Decreased liver acylglyceride accumulation in PCBP1Δhep mice fed a vit E diet. (D) Decreased expression of fatty acid genes and (E) oxidative-stress–related genes in livers of PCBP1Δhep mice fed a vit E diet. (F) Decreased LPO product 4-HNE in livers of PCBP1Δhep mice fed a vit E diet. Representative fluorescent immunohistochemistry images of liver sections stained with α-4-HNE antibody (left) and quantification (right). Data represent mean ± SD. For clarity, data for a 50-ppm iron diet without vit E are reproduced from Figs. 1, 4, and 5. Abbreviations: AU, arbitrary units; H&E, hematoxylin and eosin; ns, not significant; TAG, triacylglycerol.