Abstract
Demonstration of Trichinella spiralis in suspensions of digested muscle tissue has until now mainly been based on methods utilizing the coiling and/or the motility of the living larvae. Considering this, Jackson (1977) estimated that, compared to the number observed by examination of corresponding unfrozen samples, 78 % of larvae in frozen samples would be lost, when an ordinary pepsin-HCl digestion technique was used.
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Acknowledgment
The author wishes to thank Mr. L. Eiersted for valuable technic assistance.
References
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