Abstract
The use of a video scanning apparatus (Leitz, T.A.S.) for the determination of the corticomedullary proportion in histological sections of the avian adrenal gland is described and statistically evaluated. When the video scanning method was applied to material from groups of domestic hens, which had been exposed to different experimental conditions, the results were similar to those obtained through the integrating method as described by Siller et al. (1975). The mean values obtained by both methods did not differ significantly, and there was a highly significant correlation between the counts for both methods applied on the same sections. When applying the video scanning method to 16 sections from four adrenals, repeated measurements on each of the sections showed considerable variation. However, this variation was found to be significantly smaller than the variation among the sections. It is suggested that the video scanning method could be made more precise by improvement of the staining procedure. However, on relatively large samples it seems to give reliable results, and it has a great advantage in reducing the tedious work involved in other available methods.
Keywords: avian, adrenal, video scanning, texture analyzing system, image processing
Sammendrag
Artiklen beskriver, hvorledes et video scanning-apparat til automatisk billedanalyse (Leitz, T.A.S.) kan benyttes ved bestemmelse af proportionen af barkvaev i histologiske snit af fuglebinyrer. Metoden blev anvendt på et materiale fra grupper af tamhøner, der havde vaeret udsat for forskellige forsøgsbehandlinger, og resultaterne viste overensstemmelse med de, der kunne opnås ved integrationsmetoden beskrevet af Siller et al. (1975). Gennemsnitsvaerdierne, der opnåedes ved de 2 metoder, var ikke signifikant forskellige, og der var en meget signifikant korrelation mellem talvaerdier opnået ved begge metoder på de samme histologiske snit. Forsøg, hvor video scanning-metoden blev anvendt på 16 forskellige snit fra 4 forskellige binyrer, viste, at gentagne målinger på hvert af snittene gav betydelig variation i de opnåede talvserdier. Denne variation var dog signifikant mindre end variationen mellem de forskellige snit. Den beskrevne video scanning-metode kan formentlig gøres mere praecis ved forbedring af den anvendte farveteknik. Allerede nu kan den imidlertid, anvendt på et relativt stort materiale, give pålidelige resultater, ligesom den er hurtig og arbejdsbesparende sammenlignet med andre metoder.
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Acknowledgments
“Noco”, Copenhagen, provided free use of their T.A.S. (Texture Analysing System), and Ib Petersen, civil engineer, is thanked for his help throughout the study. Dr. W. G. Siller, The University of Edinburgh, and Dr. Erik Hasselager, The Royal Veterinary and Agricultural University, Copenhagen, are acknowledged for their help in the preparation of histological sections. The technical assistance of Birgit Jørgensen and Ellen Christiansen is highly appreciated.
References
- Bareham J R. Effects of cages and semi-intensive deep litter pens on the behaviour, adrenal response and reproduction in two strains of laying hens. Brit. vet. J. 1972;128:153–163. doi: 10.1016/S0007-1935(17)37088-4. [DOI] [PubMed] [Google Scholar]
- Bhattacharya T K, Ghosh A. Cellular modification of interrenal tissue induced by corticoid therapy and stress in three avian species. Amer. J. Anat. 1972;133:483–494. doi: 10.1002/aja.1001330409. [DOI] [PubMed] [Google Scholar]
- Chalkely H W. Methods for the quantitative morphologic analysis of tissues. J. nat. Cancer Inst. 1943;4:47–53. [Google Scholar]
- Heindle F. Der Einfluss von Oestrogenen auf die Nebenniere des Huhnes. (The influence of oestrogens on the chicken adrenal gland) 1955. [Google Scholar]
- Hohn, E. O.: Sexual behaviour and seasonal changes in the gonads and adrenals of the mallard. Proc. zool. Soc. London 1947-48, 117, 281–304.
- Kar A B. The adrenal cortex, testicular relations in the fowl: the effect of castration and replacement therapy on the adrenal cortex. Anat. Rec. 1947;99:177–197. doi: 10.1002/ar.1090990207. [DOI] [PubMed] [Google Scholar]
- Latimer H B, Landwer M F. The relative volumes and arrangement of the cortical and medullary cells of the supra-renal glands of the chicken. Anat. Rec. 1924;29:289. [Google Scholar]
- Miller R A. Regional responses of interrenal tissue and of chromaffin tissue to hypophysectomy and stress in pigeons. Acta en- docr. (Kbh.) 1967;55:108–118. doi: 10.1530/acta.0.0550108. [DOI] [PubMed] [Google Scholar]
- Miller R A, Riddle O. The cytology of the adrenal cortex of normal pigeons and in experimentally induced atrophy and hypertrophy. Amer. J. Anat. 1942;71:311–341. doi: 10.1002/aja.1000710302. [DOI] [Google Scholar]
- Oakberg E F. Genetic differences in quantitative histology of the adrenal, organ weights, and interorgan correlations in white Leghorn chickens. Growth. 1951;15:57–78. [PubMed] [Google Scholar]
- Payne F. Adrenal ganglia and medullary cells in hypophysectomized and ageing fowl. J. exp. Zool. 1955;128:259–290. doi: 10.1002/jez.1401280203. [DOI] [Google Scholar]
- Sauer F C, Latimer H B. Sex differences in the proportion of the cortex and medulla in the chicken suprarenal. Anat. Rec. 1931;50:289–295. doi: 10.1002/ar.1090500306. [DOI] [Google Scholar]
- Serra J. Theoretische Grundlagen des LEITZ-Textur-Analyse-Systems. (The theoretical basis of the LEITZ-Texture-Analyzing System) LEITZ-Mitt. Wiss. Techn. 1973;4(1):125–136. [Google Scholar]
- Siegel H S. Egg production characteristics and adrenal function in white Leghorns confined at different floor levels. Poultry Sci. 1959;38:893–898. doi: 10.3382/ps.0380893. [DOI] [Google Scholar]
- Siller W G, Teague P W, MacKenzie G M. The adrenal cortico- medullary ratio in the fowl. Brit. Poultry Sci. 1975;16:335–342. doi: 10.1080/00071667508416197. [DOI] [PubMed] [Google Scholar]
- Silverton R E, Anderson M J. Handbook of Medical Laboratory Formulae. 1961. p. 250. [Google Scholar]