Abstract
The activities of proteinases, lipases, amylases and the activities of proteinase inhibitors, as well as the numbers of Escherichia coli in the contents from the small intestine were examined for pigs suffering from colienterotoxaemia and for healthy pigs. Enzyme activities were determined using an agar diffusion test.
Compared with healthy animals the activities of proteinases and amylases in diseased animals were reduced while lipases showed increased activity. In pathologically changed contents showing large numbers of E. coli, proteinases could not be demonstrated; however, proteinase inhibitors were found in these contents. In healthy animals, proteinase inhibitors were not demonstrated in ingesta-con-taining contents.
In diseased animals, E. coli were found in large numbers in all parts of the small intestine. In healthy animals, E. coli was demonstrated especially in the posterior part of the small intestine and regularly in small numbers.
The possible influence of digestive enzymes, especially proteinases and their inhibitors, on enterotoxins from E. coli is discussed.
Keywords: digestive enzymes, intestinal contents, entero-toxaemia, pig
Sammendrag
Aktiviteten av proteinaser, lipaser og amylaser og aktiviteten av proteinase inhibitorer samt antalie t av Escherichia coli ble undersøkt i tynntarminnhold fra griser angrepet av kolienterotoksemi og sam- menholdt med tilsvarende forhold hos friske griser. Enzymaktiviteten ble besternt ved å benytte en agar diffusjonstest.
Sammenlignet med forholdet hos de friske dyrene var aktiviteten av proteinaser og amylaser redusert hos de syke dyrene, mens lipasene, derimot, hadde høyere aktivitet. I innhold som var patologisk forandret og hvor det var et høyt antall av E. coli kunne proteinaser ikke påvises. Proteinase inhibitorer ble imidlertid funnet i slikt innhold. Fra de friske dyrene ble proteinase inhibitorer ikke påvist i innhold som bestod av fórbestanddeler.
Fra syke dyr ble E. coli funnet i et stort antall i alle deler av tynntarmen. Fra friske dyr ble E. coli påvist særlig fra de bakre deler av tynntarmen og regelmessig i et lite antall.
En mulig effekt av fordøyelsesenzymer, spesielt proteinaser og deres inhibitorer, på enterotoksiner fra E. coli blir diskutert.
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