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Overview of the InVision protocol
InVision combines pre-processing steps with CUBIC clearing for clearing of the intact adult rodent eye. Enzymatic digestion of collagen rich structures using dispase allows for improved antibody permeability. Bleaching with hydrogen peroxide allows for imaging of pigmented eyes while maintaining the structural integrity of the tissue. CUBIC protocol incubation times have also been optimized for the size of the rodent eye; ON, optic nerve.
