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. 1977 Jun 1;18(2):176–186. doi: 10.1186/BF03548446

Mycoplasmas: Use of Polyvalent Antisera for Identification by Indirect Immunofluorescence

Mykoplasmer: Anvendelse af polyvalente antisera til Identifikation ved indirekte immunofluorescens

Henning Ernø 1,
PMCID: PMC8377656  PMID: 327770

Abstract

It would be an advantage, under many circumstances, to be able to make use of polyvalent antisera in the process of identifying mycoplasmas. As the indirect immunofluorescence test is sufficiently sensitive and also generally accepted as being rather specific, this technique was chosen to investigate whether polyvalent antisera are applicable in routine identification of mycoplasmas. Three polyvalent sera were used, each consisting of 9 or 10 rabbit antisera raised against 29 of the more common species of the genus Mycoplasma. Twenty-six field strains were examined. One strain did not react with any of the 3 polyvalent antisera although it was later identified as M. bovigenitalium. The remaining 25 strains reacted with 1 and only 1 of the polyvalent antisera and were subsequently identified by immunofluorescence utilizing monospecific antisera. Strains of the following species were identified: M. arginini, M. bovigenitalium, M. bovis, M. bovoculi, M. canis, M. capricolum, M. cynos, M. edwardii, M. hominis, M. hyorhinis, M. molare, M. mycoides subsp. mycoides and M. opalescens. It is concluded that polyvalent antisera may be used in identification procedures and thereby permit the use of a limited number of monospecific antisera without preceding biochemical testing.

Keywords: mycoplasmas, identification, indirect immunofluorescence, polyvalent sera

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References

  1. Ernø H, Stipkouits L. Bovine mycoplasmas: Cultural and biochemical studies. I. Acta vet. scand. 1973;24:436–449. doi: 10.1186/BF03547431. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Emø H, Jurmanouá K, Leach R H. Bovine mycoplasmas: A serological study by the metabolic inhibition test. Acta vet. scand. 1973;14:511–523. [PubMed] [Google Scholar]
  3. Lagercrantz M, Espmark A. Typing of enteroviruses by neutralization tests using combinatorial antiserum pools applied in filter paper discs. J. biol. Stand. 1973;1:237–245. doi: 10.1016/0092-1157(73)90018-8. [DOI] [Google Scholar]
  4. Polak-Vogelzang A A, Hagenaars R. Identification of mycoplasmas and acholeplasmas by means of an indirect immunoperoxidase test. Proc. Soc. Gen. Microbiol. 1976;3:151. doi: 10.1099/00221287-106-2-241. [DOI] [PubMed] [Google Scholar]
  5. Rosendal S, Black F T. Direct and indirect immunofluorescence of unfixed and fixed mycoplasma colonies. Acta path, microbiol. scand. Sect. B. 1972;80:615–622. doi: 10.1111/j.1699-0463.1972.tb00186.x. [DOI] [PubMed] [Google Scholar]
  6. Schmidt N J, Melnick J L, Wenner H A, Ho H H, Burk-hardt M A. Evaluation of enterovirus immune horse serum pools for identification of virus field strains. Bull. Wld Hlth Org. 1971;45:317–330. [PMC free article] [PubMed] [Google Scholar]
  7. Shepard M C, Howard D R. Identification of “T” mycoplasmas in primary agar cultures by means of a direct test for urease. Ann. N.Y. Acad. Sci. 1970;174:809–819. doi: 10.1111/j.1749-6632.1970.tb45598.x. [DOI] [PubMed] [Google Scholar]

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