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. 1977 Sep 1;18(3):323–334. doi: 10.1186/BF03548430

Glutathione Peroxidase Activity in Porcine Blood

P Fogd Jørgensen 1,✉, J Hyldgaard-Jensen 1, J Moustgaard 1
PMCID: PMC8377698  PMID: 910656

Abstract

Determination of the seleno-enzyme glutathione peroxidase (GSH-Px) in blood from Danish Landrace pigs was done using a quantitative, spectrophotometric method and a simple “spot test”. A close correlation between the net reaction rate measured spectrophotometrically (Δ A/min.) and time for defluores-cence (minutes) was obtained (r2 = 0.72—0.77, P < 0.0005). From these results the factors used for a conversion of defluorescence time to u/g hemoglobin were evaluated. The results further showed that the “spot test” can be used as a screening method for detection of subnormal GSH-Px levels in pigs.

While red cell GSH-Px seems independent of the sex, an elevation of both plasma and red cell GSH-Px was found with increasing age of pigs. The normal range of red cell GSH-Px activity was wide, contrasting the small variations observed in the individual pig. Some evidence that porcine red cell GSH-Px is under genetical control was found and discussed in relation to the possible use of GSH-Px as an indicator of the pig's selenium status.

Keywords: pigs, glutathione peroxidase, spectrophotometric determination, spot test, erythrocytes, plasma

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