Fig. 3.

Determination of the Env regions required for 1E5 binding. a Schematic presentation of the Env recombinants constructed between REJO4541.67 (SVPB16), which is not recognized by 1E5, and 93TH976.17, which is strongly bound by 1E5. The regions from REJO4541.67 and 93TH976.17 are shown in light-blue and red, respectively. The W69G mutation is shown in yellow. Deletion in the V1, V2 and V1V2 region was shown by a dotted line. b The reactivity of Env recombinants to NHG, VRC01 and 1E5 was determined by flow cytometry analysis using cells expressing each Env recombinant. The reactivity was detected by APC-conjugated anti-human IgG secondary Ab, and the mean fluorescence intensity (MFI) of APC is shown. Experiments were performed three times, and the representative result is shown