Fig. 4.

Validation of the glyco-signature on Puleo patient's cohort. (a) Two-dimension representation of the HCPC analysis results and (b) cluster dendrogram related to affymetrix transcriptomic data (n = 309) using 19 GT genes. (c) Survival curves estimated by using the Kaplan-Meier method and comparing OS probabilities between clusters with the log-rank test (p-value = 0.0045). Cluster 1 and 2 have shorter median OS of 23.8 and 22.9 months, respectively compared to cluster 3 with median OS of 46.4 months. (d) Mosaic plot showing cross-link between basal-like/classical subtypes according to PurIST classifier and identified clusters 1, 2 and 3 through GT gene prognostic markers. Box height reflects the number of tumors classified in each cluster and box width represents proportion of basal-like/classical subtypes (p-value = 3.597e-05, Chi-squared test). (e) Mosaic plot showing the microenvironment subtype proportion in clusters 1, 2 and 3 through GT gene prognostic markers, related to microenvironment-based classification of PDAC tumors, proposed by Puleo et al. [12]. Box width reflects the number of tumors classified in each cluster and box height represents proportion of microenvironment subtypes (p-value < 0.0001, Chi-squared test). For both mosaic plots, the standardized residuals, with an absolute value greater than 2.0, indicate boxes contributing to significant chi-square test statistic. The color range from red to blue indicates whether observed frequency is significantly higher or lower, respectively than the expected frequency (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article).