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. 2021 Aug 12;148(16):dev199686. doi: 10.1242/dev.199686

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© 2021. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — Germ cell Fis1 deletion results in multinucleated spermatid giant cells. (A) PAS staining of adult testis sections, counterstained with Hematoxylin. Note the large, multinucleated GCs in S8/Fis1 testes. Scale bar: 20 µm. (B) Immunohistochemical staining of testis sections with an antibody against the spermatid-specific SP-10 protein. The acrosome in a control round spermatid is indicated by a white arrow. Spermatids in mutant GCs lack acrosomes and stain intensely for SP-10. Scale bar: 20 µm. (C) Quantification of the number of GCs with SP-10 reactivity. (D) Quantification showing the prevalence of spermatid GCs in Fis1 mutants. Control testes never exhibit GCs. All data are from adult (P56) mice. Data are represented as mean±s.e.m. ****P≤0.0001. For statistical tests used, see the Materials and Methods section.