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RT-qPCR was performed on nasal, oropharyngeal, and rectal swabs collected from principal infected (A) and sentinel deer (B) on the indicated days post challenge (DPC). Mean (n=2) viral RNA copy number (CN) per mL of the SARS-CoV-2 nucleocapsid gene is reported. The limit of detection for this assay is indicated by the dotted lines. Asterisks (*) indicate samples with 1 out of 2 RT-qPCR reactions below the limit of detection.
