Table 7.

Absolute concentration of metabolic compounds in WT and KO cells.

	WT Mean concentration (n = 3) (pmol/106cells)		KO Mean concentration (n = 3) (pmol/106cells)		KO vs WT
Compound name	Mean	S.D.	Mean	S.D.	Ratio a	p-value b
Glucose 6-phosphate	34	6.0	38	1.2	1.1	0.340
Fructose 6-phosphate	11	4.5	12	0.7	1.1	0.712
Fructose 1,6-diphosphate	247	127	373	43	1.5	0.221
Glyceraldehyde 3-phosphate	34	25	31	5.9	0.9	0.880
Dihydroxyacetone phosphate	143	78	147	32	1.0	0.933
Glycerol 3-phosphate	69	19	64	6.6	0.9	0.723
2,3-Diphosphoglyceric acid	17	1.4	18	0.4	1.1	0.381
Valine	2541	273	1896	166	0.7	0.034 *
Leucine	2176	211	1581	137	0.7	0.020 *
Isoleucine	2346	260	1681	121	0.7	0.031 *
Tryptophan	182	12	71	6.3	0.4	8.6E-04 ***
Lysine	1041	127	1102	36	1.1	0.497
ATP	5270	547	5336	297	1.0	0.867
ADP	777	67	436	33	0.6	0.005 **
AMP	156	16	64	4.7	0.4	0.006 **
GTP	927	72	906	73	1.0	0.743
GDP	106	6.6	59	2.1	0.6	0.004 **
GMP	25	2.6	12	0.9	0.5	0.008 **
NAD+	722	83	711	74	1	0.871
NADH	64	19	45	6.2	0.7	0.226

^aThe ratio is computed by using averaged detection values. The latter was used as denominator.

^bThe p-value is computed by Welch’s t-test (*<0.05, ** <0.01, *** <0.001)