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SRM image of the sample (scale bar: 15 μm). (a) Oil was stained with Nile red, and (b) WPNFs were stained with rhodamine isothiocyanate B. (c) Microscope images were obtained to show the overlapping fluorescent fields from (a,b). Nile red and rhodamine B isothiocyanate were simultaneously fluorescently excited at 488 nm and 568 nm, shown as green and red in the image, respectively.
