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Effects of metformin on the generation of reactive oxygen species (ROS). We evaluated the effects of increasing doses of metformin on the production of mitochondrial (A) and cellular (B) ROS in human brain microvascular endothelial cells cultured at the indicated conditions (Glu: glucose; Mann: mannitol). *: p < 0.05 vs. Glu 5 mM; #: p < 0.05 vs. Vehicle.
