Figure 3.

Validation of gip2-dependent hub and bottleneck genes. Downregulation of Hub and Bottleneck node genes upon silencing of gip2 was validated using SKOV3 (A), OVCAR8 (B) and OVCAR3 (C) cells in which the expression of gip2 was silenced using specific shRNA (SKOV3) or siRNAs (OVCAR8 and OVCAR3). In the case of SKOV3 cells, cells expressing non-targeting scrambled RNA were used as control group (shCON). In OVACR8 and OVCAR3 cell, cells transfected with non-targeting scrambled siRNA pool were used as the control group (SiCON). Downregulated genes were validated by monitoring the expression of the representative bottleneck gene IL6 and hub gene UQCRC1. Statistical significance was determined by Student’s t-test (* p < 0.05, ** p < 0.005, *** p < 0.0005, **** p < 0.0001).