Figure 2.

Effect of H₂O₂ on mRNA expression and reporter activity of TRPM6. (A) NRK-52E cells were treated with vehicle (CNT), 10 nM insulin, and 500 μg/mL GA for 1 h. The intracellular ROS contents were measured using DCF and represented as percentage of CNT. (B) Cells were treated in the presence or absence (CNT) of 500 μg/mL GA and 2 mM NAC for 6 h. The mRNA levels of TRPM6, TRPM7, and CNNM2 were represented as percentage of CNT. (C) Cells were treated with H₂O₂ for 6 h at the concentrations indicated. The mRNA levels of TRPM6, TRPM7, and CNNM2 were represented as percentage of 0 μM. (D) Cells were treated with vehicle (CNT) and 50 μM H₂O₂ for 1 h. The expression levels of p-ERK and ERK were measured by Western blotting and represented as percentage of CNT. (E) The reporter vector of TRPM6 was transfected into the cells using HilyMax. The transfection efficiency was corrected for by the ratio of firefly luciferase activity to Renilla luciferase activity and represented as percentage of CNT. n = 3–5. ** p < 0.01, * p < 0.05 and ^NS p > 0.05 compared with CNT or 0 μM. ^## p < 0.01 compared with -NAC.