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. 2021 Jul 26;10(8):1893. doi: 10.3390/cells10081893

Figure 4.

Figure 4

Effects of TRPM6 expression modulators on the elevation of [Mg2+]i. (A) NRK-52E cells were treated in the absence (CNT) or presence of 500 μg/mL GA, 2 mM NAC, and 50 μM H2O2 for 24 h. [Mg2+]i was measured using a fluorescence microplate reader. The extracellular solution was changed to HBSS containing 5 mM MgCl2 at 0 min. [Mg2+]i was measured using a fluorescence microplate reader and represented as percentage of 0 min. (B) The cells transfected with si-Neg or si-miR-24-3p were treated with 50 μM H2O2 for 24 h. [Mg2+]i was represented as percentage of 0 min. n = 5–6. ** p < 0.01 compared with CNT. ## p < 0.01 compared with GA. NS p > 0.05 compared with CNT or si-miR-24-3p.