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. 2021 Aug 18;10(8):2126. doi: 10.3390/cells10082126

Figure 4.

Figure 4

Repopulation of decellularized ovarian ECM-based scaffolds with pOCs. (a) Image illustrating freshly isolated pOCs. Scale bar = 100 μm. (b) pOCs adhered and migrated into the decellularized ECM-based ovarian scaffolds. Representative image after 7 days of culture. Scale bar = 100 μm. (c) H & E staining demonstrated the presence of pOCs in the ECM-based scaffolds. Scale bar = 100 μm. (d) DAPI staining confirmed the positivity for nuclei. Scale bar = 100 μm. (e) Cell density analysis showed ECM-based scaffolds’ repopulation after 24 h, with a higher number of cells after 48 h and 7 days of coculture. Data are expressed as the mean ± the standard error of the mean (SEM). a and b indicate statistically significant differences (p < 0.05). (f) DNA quantification demonstrated the presence of pOCs into the ECM-based scaffolds after 24 h, with an increasing cell number at 48 h, which was steadily maintained at Day 7. Data are expressed as the mean ± the standard error of the mean (SEM). a and b indicate statistically significant differences (p < 0.05). (g) Representative picture of the TUNEL assay showing positive cells in red. Nuclei were stained with DAPI (blue). Scale bars = 100 μm and 50 μm. (h) TUNEL-positive cell rates detected 24–48 hours and 7 days postseeding were comparable to those identified in the native tissues (native). Data are expressed as the mean ± the standard error of the mean (SEM).