Figure 3.

Effects of rMSA on the spatial learning and memory of mice. (a,b) Measurements of the percentage of successful escape (a) and the primary escape latency (b) of male mice. (c) The average of (a). n, the number of trials. (d) The average of (b). n, the number of trials. (e) The representative images of p-tau in the mice cortex. Scale bar, 50 μm. (f–h) The quantitative results of p-tau (green) and DAPI (blue) in male (f), female (g) and female + male (h) mice. n, the number of mice used for analysis. Mice were treated with rMSA 1.5 mg per gram of body weight or isometric saline every 3 weeks for 8 months. All graphs represent mean with SEM, with p values calculated by the two-tail t test.