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. 2021 Aug 20;10(8):2152. doi: 10.3390/cells10082152

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Expression of human transgenes in porcine chondrocytes. (A) Representative RT-PCR of WT and genetically modified porcine chondrocytes regarding human CD46, CD55, CD59, TNFAIP3; porcine GAPDH served as control (shown in Figure 1). The corresponding samples were loaded for electrophoresis in the following order: lane M: bp marker; lane 1: WT; lane 2: TG; lane 3: GalTKO; lane 4: GalTKO/TG; lane 5: GalT/CMAHKO; lane 6: GalT/CMAHKO/TG; NC: negative control (B) HMOX1 gene expression in human (hCH) and porcine chondrocytes (WT, TG and GalTKO/TG).