Effect of orlistat on NF-κB p65 target protein levels in WD-driven CAC mice. (a) Immunoreactivity of NF-κB p65; (b) estimated immunoscores for NF-κB p65; (c) p-p65, IκB, and pIκB expression; (d) p-p65, IκB, and pIκB relative protein expression levels; (e) NF-κB-related genes in colonic tissue were evaluated by western blot analysis; (f) XIAP, Cdk4, Cyclin D, and Bcl-2 relative protein levels. Relative protein expression levels were calculated by densitometric analysis and normalized to β-actin. #
p < 0.05 and ###
p < 0.001 when compared to Con; * p < 0.05, ** p < 0.01, and *** p < 0.001 when compared to WD + AOM/DSS.