Figure 7.

Cytosolic Ca²⁺ transient in primary hippocampal neurons induced by glutamate. Panel (A), representative image of 14 div hippocampal neurons expressing cytoplasm-targeted jrGECO. Panel (B), representative traces of jrGECO fluorescence expressed as the normalized values of the difference between time point F minus the average of the initial 5 min of acquisition (F₀); where indicated the cells were challenged with 2 μM glutamate and 30 mM KCl. Panel (C), quantification of the jrGECO fluorescence measured at the maximum peak reached after glutamate addition and expressed as normalized difference with initial fluorescence F₀. WT n = 144 cells, median value = 4.13; B6.152H n = 121 cells, median value = 4.2. Two sample t-test prob. = 0.9; at the 0.05 level, the difference between the means is not significantly different from 0. Panel (D), jrGECO fluorescence measured 20 min after glutamate addition and expressed as before. WT n = 144, median value = 2.33; B6.152H n = 121 cells, median = 2.24; Mann–Whitney test Asymp prob = 0.1. At the 0.05 level, the two distributions are not significantly different. Box plot: Box height, 25–75%; whiskers, 1–99%; white square: Mean; transverse line: Median; curve: Normal distribution.