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. 2021 Aug 14;10(8):2093. doi: 10.3390/cells10082093

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Assessing CEACAM1 expression in endothelial cells. (A) Primary cells were isolated from male mice at 2 months of age (n = 5/genotype), except for hepatic stellate cells that were derived from male mice at 8 months of age. Ceacam1 mRNA levels were analyzed by qRT-PCR in triplicate and normalized to 18S. Values are expressed as mean ± SEM. * p < 0.05 vs. all control groups; Negl, negligible. (B). Liver endothelial cells (LEC) combined from several wild-type (VECad-Cc1^+/+) and null (VECad+Cc1^fl/fl) mice were analyzed by immunoblotting (Ib) with specific antibodies (α-) to detect specific proteins and normalized against levels of loaded proteins by immunoblotting parallel gels or lower half-gels with α-GAPDH or α-Tubulin. The apparent molecular mass (kDa) is indicated at the right-hand side of each gel. Gels represent two separate/repeated experiments.

Assessing CEACAM1 expression in endothelial cells. (A) Primary cells were isolated from male mice at 2 months of age (n = 5/genotype), except for hepatic stellate cells that were derived from male mice at 8 months of age. Ceacam1 mRNA levels were analyzed by qRT-PCR in triplicate and normalized to 18S. Values are expressed as mean ± SEM. * p < 0.05 vs. all control groups; Negl, negligible. (B). Liver endothelial cells (LEC) combined from several wild-type (VECad-Cc1^+/+) and null (VECad+Cc1^fl/fl) mice were analyzed by immunoblotting (Ib) with specific antibodies (α-) to detect specific proteins and normalized against levels of loaded proteins by immunoblotting parallel gels or lower half-gels with α-GAPDH or α-Tubulin. The apparent molecular mass (kDa) is indicated at the right-hand side of each gel. Gels represent two separate/repeated experiments.