Table 1.
mRNA levels of genes in primary liver endothelial cells.
| VECadCre−Cc1+/+ | VECadCre+Cc1fl/fl | |
|---|---|---|
| Cell adhesion | ||
| Vcam1 | 1.51 ± 0.11 | 6.24 ± 0.55 * |
| Icam1 | 1.01 ± 0.19 | 5.34 ± 0.15 * |
| P-Selectin | 1.34 ± 0.12 | 3.72 ± 0.57 * |
| Adherence/Tight junctions | ||
| Vecadherin | 5.12 ± 1.28 | 2.15 ± 0.43 * |
| β-Catenin | 7.23 ± 1.42 | 3.43 ± 0.87 * |
| Occludin | 6.75 ± 1.22 | 3.21 ± 0.51 * |
| ZO-1 | 3.75 ± 0.56 | 1.04 ± 0.19 * |
| Claudin1 | 4.68 ± 1.13 | 1.52 ± 0.76 * |
| Claudin5 | 4.89 ± 1.31 | 1.67 ± 0.64 * |
| Vascular permeability | ||
| Vegf-A | 8.37 ± 1.68 | 2.45 ± 0.78 * |
| Vegf-C | 1.56 ± 0.07 | 1.41 ± 0.09 |
| Vegf-D | 0.98 ± 0.12 | 0.95 ± 0.11 |
| Vegfr1 | 2.01 ± 0.04 | 0.97 ± 0.03 * |
| Vegfr2 | 1.75 ± 0.08 | 0.37 ± 0.06 * |
mRNA levels were assessed in liver endothelial cells from 2-month-old male mice (n = 5/genotype) by qRT-PCR in triplicate using gene-specific primers and normalized to 18S. Values are expressed as mean ± SEM. * p < 0.05 vs. VECadCre−Cc1+/+.