Abstract
The agarose gel electrophoresis described by Johansson (1972) was modified so that a buffer of pH 7.9 was used in the gel, whereas the buffer in the electrode vessels had a pH of 8.6.
The cattle blood serum protein picture is described in detail. The β1-globulin zone shows a very distinct picture of the genetically polymorphic bovine transferrins. The region between the α- and β-globulins shows a number of faint and often very distinct bands. A faint background staining over the whole electrophoretogram may partly be caused by a rather strong lipoprotein in the α1-region, lipids thus having migrated all over the electrophoretogram.
The modified method described is well suited as a “screen electrophoresis” for cattle serum and is also useful e.g. in studying bovine transferrin polymorphism.
Keywords: electrophoresis, agarose gel electrophoresis, screen, bovine serum, cattle
Sammanfattning
Den agarosgelelektrofores som beskrivits av Johansson (1972) har modifierais så att buffert med pH 7,9 användes i gelet, medan bufferten i elektrodkärlen är av pH 8,6. Med denna och några andra, smärre modifikationer har metoden blivit lämplig för “screen”-elektroforetiska undersökningar av blod från nötkreatur.
Den serumproteinbild som erhålles hos nötkreatur med denna metod beskrivs i detalj. β1-globulin-zonen uppvisar en mycket distinkt bild av transferrinpolymorfismen hos nötkreatur. Området mellan α- och β-globulin visar ett antal svaga men ofta mycket distinkta proteinband. En svag bakgrundsfärgning över hela elektroforetogrammet kan möjligen till viss del orsakas av ett ganska starkt α1-lipoprotein, där sälunda lipoider migrerat hela vägen över elektroforetogrammet.
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Footnotes
The investigations were supported by the Swedish Council for Forestry and Agricultural Research.
References
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