Abstract
On the basis of biochemical and serological criteria 2 hemolysin forming varieties of peptococci were identified as Peptococcus indolicus.
Of a total of 16 hemolytic strains examined 9 originated from the vagina of clinically healthy cows, 4 from mastitis secretions from dry cows, 2 from the interdigital skin of clinically healthy sows, and 1 from a subcutaneous abscess in a pig.
Two strains were designated a-hemolytic and 14 β-hemolytic.
On blood agar plates colonies of the α-hemolytic variety were surrounded by narrow zones of almost complete hemolysis, while colonies of the β-hemolytic variety were surrounded by broad zones of incomplete hemolysis. The hemolysins were termed α- and β-hemolysin, respectively.
The β-hemolysin, but not the α-hemolysin, could be demonstrated in cultures grown in liquid media. The β-hemolysin was found to be filtrable, relatively thermoresistant, and non-dermonecrotic.
By gel diffusion analyses the 2 α-hemolytic strains were referred to Serotype C. Ten of the β-hemolytic strains belonged to Serotype C, 2 to Type B, 1 to Type D, and 1 to Type E.
Keywords: peptococcus (s. micrococcus) indolicus, hemolysin formation
Sammendrag
To hæmolysindannende varianter af peptokokker blev på grundlag af biokemiske og serologiske kriterier fundet identiske med Peptococcus (s. Micrococcus) indolicus.
Af ialt 16 undersøgte stammer var 9 isoleret fra klinisk sunde køers vagina, 4 fra mastitis sekreter fra goldkøer, 2 fra huden i klovspalten hos klinisk sunde søer og 1 fra en subkutan absces hos en gris.
På blodagar plader var den ene variants kolonier omgivet af smalle, næsten klare hæmolysezoner (fig. 1). Denne variant foreslås betegnet α-hæmolytisk. Den anden variant, som blev betegnet β-hæmolytisk, dannede kolonier, omgivet af brede zoner med ufuldstændig hæmolyse (fig. 2). Hæmolysinerne blev betegnet henholdsvis α- og β-hæmolysin.
To stammer var α-hæmolytiske, 14 var β-hæmolytiske.
β-hæmolysinet kunne påvises i kulturer i kødvands pepton bouillon og i Robertson’s cooked meat medium, hvorimod α-hæmolysinet ikke blev påvist i sådanne kulturer.
β-hæmolysinet fandtes at være filtrerbart, relativt termoresistent og non-dermonekrotisk.
Ved gel diffusionsanalyse fandtes de 2 α-hæmolytiske stammer at være af serotype G. Ti β-hæmolytiske stammer var af serotype C, 2 af type B, 1 af type D og 1 af type E.
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