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. 2021 Jul 29;13(8):2608. doi: 10.3390/nu13082608

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Curcumin significantly prevented hemodynamic overload-induced acetylation of GATA4 in DS rats. (A) Nuclear extracts (NE) of LV tissues in each rat were subjected to western blotting with indicated antibodies. (B,C) The protein levels of p300 (B) and GATA4 (C) were normalized by β-actin. (D) The immunoprecipitated samples with anti-GATA4 antibody were subjected to western blotting with indicated antibodies. (E) The acetylated level of GATA4 was normalized to total GATA4. The band intensities were quantified using Image J v1.47 software. The values are represented as the mean ± SEM. All values were normalized to DR vehicle group, which was set at 1.0 as the control.