Effect of human serum enriched with ALE metabolites on cholesterol and triglyceride levels in HepG2 cells. HepG2 cells were preincubated with fetal calf serum for 24 h and stimulated with 250 µM palmitate or vehicle for an additional 48 h (A,C). To analyze the effects of human serum enriched with metabolites, cells were preincubated for 24 h in DMEM in the presence of 10% of human enriched serum according to the Clinic’n’Cell protocol (DIRV INRA 18-00058) prior to an additional 48 h treatment palmitate (Sigma, St. Louis, MO, United States) at 250 µM. Intracellular triglycerides (A,B) and total cholesterol were measured (C,D). Palmitate stimulated HepG2 triglyceride and cholesterol content independently of the origin of the serum (calf or human). The human serum enriched with ALE metabolites significantly limited the intracellular cholesterol levels. * (p < 0.05); ** (p < 0.01); ns: no significant difference.