Table 2.

HPLC calibration curves, detection limit, and quantitation limit for HCQS from 6.25 to 1000 µg/mL.

	Regression Equation	Detection Limit (µg/mL)	Quantitation Limit (µg/mL)
Deionised water	A = 43,294C + 32,746	2.7	8.3
50:50 v/v methanol:water	A = 42,848C − 19,037	7.2	21.7

A = Peak area at 254 nm. C = HCQS concentration in µg/mL. The slopes and y-intercepts were the mean of 11 and 14 values for water and the co-solvent, respectively.