Figure 6.

The effect of ATP-citrate lyase deletion on TAG synthesis on an S medium containing glucose and acetate. Symbols: closed circles, ∆lslig4; closed squares, ∆lslig4∆acl1∆acl2; gray bars, ∆lslig4; black bars, ∆lslig4∆acl1∆acl2. N.D. indicates not detected. (A–E) The analysis of cell concentration, glucose concentration, TAG concentration, amount of TAG per cell, and relative expression levels of genes involved in acyl-CoA synthesis were performed as described in Figure 5, except that the S medium containing glucose was replaced with an S medium containing glucose and acetate. Asterisks indicate statistically significant differences between ∆lslig4 and ∆lslig4∆acl1∆acl2 (t-test; * p < 0.05).