Table 1.
C. perfringens strains used in this study.
| Strain ID (Alternative Name) | NetB d | netB Sequence a | Description | Origin | Reference |
|---|---|---|---|---|---|
| JIR4869 (EHE-NE18) | + | Con b | Necrotic enteritis, broiler | Australia | [25] |
| JIR12071 (NE18 Δα, NE18-M1) | + | Con b | Alpha toxin mutant from strain JIR4869 | Australia | [25] |
| JIR12331 (NE18 ΔnetB) | − | NetB toxin mutant from strain JIR4869 | Australia | [13] | |
| JIR4860 (EHE-NE5) | + | Con b | Necrotic enteritis, broiler | Australia | [25] |
| JIR4866 (EHE-NE15) | + | Con b | Necrotic enteritis, broiler | Australia | [25] |
| JIR12058 (UNK-NE30) | + | G502A (A168T) c | Necrotic enteritis, broiler | Australia | [23] |
| JIR4857 | − | Necrotic enteritis, broiler | Australia | [26] | |
| CP3 | − | Healthy broiler | Belgium | [27] | |
| CP4 | − | Healthy broiler | Belgium | [27] | |
| CP23 | + | G502A (A168T) c | Healthy broiler | Belgium | [14] |
| CP56 (JIR12037) | + | Con b | Necrotic enteritis, broiler | Belgium | [27] |
| D3 (99.63206-34) | + | G502A (A168T) b | Necrotic enteritis, broiler | Denmark | [23] |
| S2 | − | Necrotic enteritis, broiler | Denmark | [28] | |
| S36 | + | G502A (A168T) c | Necrotic enteritis, broiler | Denmark | - |
| JGS4100 | + | G502A (A168T) c | Necrotic enteritis, broiler | USA | G. Songer, pers.com. |
| JGS4121 | − | Necrotic enteritis, broiler | USA | G. Songer, pers. com. |
a C. perfringens netB sequence. Con: EHE-NE18 netB consensus sequence (EU143239); G502A (A168T): netB sequence with a nucleotide change at position 502 of the coding sequence (Change from Ala to Thr at amino acid position 168 of the NetB protein). b C. perfringens netB sequence reported by Keyburn et al. 2010 [23]. The nucleotide substitution reported by Keyburn et al. was numbered relative to the start of the sequencing place (i.e., 267 bp upstream of the netB start codon), resulting in G769A. This corresponds to position 502 of the netB gene reported by Abildgaard et al. 2010 and this study [24]. c C. perfringens netB sequence obtained in this study. d Distinction between NetB-positive and NetB-negative C. perfringens strains. +: NetB-positive C. perfringens strain. −: NetB-negative C. perfringens strain.